mouse col1 elisa kit  (Elabscience Biotechnology)

Journal: Theranostics

Article Title: Nanodelivery of Y-27632 by RGD-modified liposome enhances radioimmunotherapy of hepatocellular carcinoma via tumor microenvironment matrix stiffness reprogramming

doi: 10.7150/thno.114892

Figure Lengend Snippet: Preparation and characterization of RGD@LP-Y. ( A ) Schematic illustration of RGD@LP-Y preparation. ( B ) TEM observation of RGD@LP-Y morphology. ( C ) Zeta potential of RGD@LP-Y. ( D ) The cumulative release of LP-Y and RGD@LP-Y at different pH values (pH 7.4 and 5.5). ( E ) Variations in the particle size of RGD@LP-Y under conditions of 4 ℃, -20 ℃, -80 ℃, and 10% serum after 12 days. ( F ) Relative viability of Hepa1-6 cells treated with different liposomes measured by CCK-8 assay. ( G ) mRNA expression levels of YAP and COL1A2. ( F ) Detection of COL1 level in Hepa1-6 cell supernatant by ELISA. LP, liposomes without RGD modification; RGD@LP, RGD-modified liposomes; LP-Y, liposomes encapsulating Y-27632 but without RGD modification; RGD@LP-Y, RGD-modified liposomes encapsulated with Y-27632. Values represent mean±SD. *P < 0.05, **P < 0.01, ***P < 0.001. ns: not significant.

Article Snippet: The Mouse IL-12 (Interleukin 12) ELISA Kit [E-EL-M3062], Mouse COL1 (Collagen Type I) ELISA Kit[E-EL-M0325], Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit [E-EL-H0109], Human IL-6 (Interleukin 6) ELISA Kit [E-EL-H6156], Human IL-1β (Interleukin 1 Beta) ELISA Kit [E-EL-H0149], Mouse IL-6 (Interleukin 6) ELISA Kit [E-EL-M0044], and Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit [E-EL-M3063] were purchased from Elabscience Biotechnology Co., Ltd. (China).

Techniques: Zeta Potential Analyzer, Liposomes, CCK-8 Assay, Expressing, Enzyme-linked Immunosorbent Assay, Modification

Journal: Cellular & molecular biology letters

Article Title: New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling.

doi: 10.1186/s11658-024-00614-5

Figure Lengend Snippet: Fig. 6 The regulatory effect of P2X7 receptor via the PI3K/AKT/GSK3β signaling pathway in vivo. A Western blot analysis measured the phosphorylation levels and grayscale values of P13K, AKT, and GSK3β proteins in mouse femur tissue from each group; B immunohistochemistry staining assessed the expression and positive area percentage of p-P13K, p-AKT, and p-GSK3β in mouse femur tissue of each group; C bar graph presenting the number of TRAP-positive cells in mouse femur tissue sections stained with TRAP; D western blot analysis determined the expression levels and band grayscale values of MMP9, NFATc1, and CK proteins in mouse femur tissue from each group; E immunohistochemistry staining assessed the expression and positive area percentage of MMP9 and NFATc1 in mouse femur tissue; F ELISA measured the expression levels of CTX and NTX in mouse serum; G Von Kossa staining assessed the calcium salt deposition in mouse femur tissue sections. Bar = 100 μm. Compared with the WT + Sham group, ***P < 0.001; compared with the WT + OVX group, ###P < 0.001; compared with the KO + OVX group, &&&P < 0.001

Article Snippet: We used a mouse Cross-linked Type I Collagen C-Telopeptide (CTX) ELISA kit (M3023, Elabscience, Wuhan, China) and a mouse Cross-linked Type I Collagen N-Telopeptide (NTX) ELISA kit (E-EL-M3022, Elabscience, Wuhan, China) for mice.

Techniques: In Vivo, Western Blot, Phospho-proteomics, Immunohistochemistry, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell Communication and Signaling : CCS

Article Title: SHH induces macrophage oxidative phosphorylation and efferocytosis to promote scar formation

doi: 10.1186/s12964-024-01692-w

Figure Lengend Snippet: SHH promoted collagen deposition and polarization of M2 macrophage. A , wound model of dorsal skin was established in C57BL/6 mice, and the wound was treated with rmSHH or vector. The wound size during the healing period was recorded and measured ( n = 6). B , masson staining was performed and the dermal layer were measured to evaluate the collagen deposition of the wound bed. C , ELISA was performed to measure the expression of type I collagen of the wound. D , IHC of CD31 was performed to compared the neovascularization of the two group. E-G , IF of Ly6G, F4/80, iNOS and CD206 were performed to evaluate the infiltration of neutrophil (E) , M1 (F) and M2 macrophage (G) . H , flow cytometry was performed to evaluate the macrophage phenotype of the wound bed. * p < 0.05, ** p < 0.01, n = 6

Article Snippet: Mouse COL1 ELISA Kit (No.D721060, Sangon Biotech, China) was used for COL1 determination of the wound area.

Techniques: Plasmid Preparation, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry

Journal: Cell Communication and Signaling : CCS

Article Title: SHH induces macrophage oxidative phosphorylation and efferocytosis to promote scar formation

doi: 10.1186/s12964-024-01692-w

Figure Lengend Snippet: Vismodegib alleviated scar formation by targeting SHH signal. A-D , OCR, maximal respiration response, complex I, II and ATP production of macrophage treated with vismodegib were measured ( n = 3). E , apoptotic neutrophil was marked with PKH26 and incubated with bone marrow derived macrophage. Flow cytometry was performed to evaluate the efferocytosis of macrophage treated with vismodegib ( n = 3). F-J , wound model of dorsal skin was established in C57BL/6 mice, and vismodegib was administrated ( n = 6). F , masson staining was performed and the dermal layer were measured to evaluate the collagen deposition of the wound bed. G , IF and ELISA was performed to evaluate the expression of type I collagen. H-I , IF of F4/80, iNOS and CD206 were performed to evaluate the macrophage infiltration of wound bed. J , flow cytometry was performed to evaluate the phenotype of macrophage from wound bed treated with vismodegib. * p < 0.05, ** p < 0.01

Article Snippet: Mouse COL1 ELISA Kit (No.D721060, Sangon Biotech, China) was used for COL1 determination of the wound area.

Techniques: Incubation, Derivative Assay, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Expressing